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publicationDate 2010-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2010002636-A1
titleOfInvention Assays for nuclear hormone receptor binding
abstract Methods and genetic constructs are provided for detecting the binding of nuclear hormone receptors to a coactivator/corepressor. The methods employ enzyme fragment complementation using fragments of β-galactosidase as the detection system. Cells are transformed to express the large fragment of β-galactosidase fused to a member of the complex with NHR for initiation of transcription and have it localized in the nucleus and to express the small fragment of β-galactosidase fused to the nuclear hormone receptor for binding to the member upon stimulation with a ligand.
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