| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7009c1a23b90d251e276dbaa39f2c559
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4032d4610f4b8faa658596427ae7243a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_64bdf2dd6314dd1afdc0f29354aee50f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ccae31f709cbe5b67fac7ac4fc19902b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2efbe95965e682e179dfd266c9980505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_658da3351c95cae75f955712944c5e22 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate |
2009-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e3feb57aaa645a02728ec8e777e9107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6368de29b4f5baf912d22dee7ca4d78d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b1493bdc6c9536ad0880e0fc47ad0a3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3b62c178177ab4a4f0b78fc0cbcf849
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79ea1b7141564d2860e4894b1e619c68 |
| publicationDate |
2010-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009158139-A3 |
| titleOfInvention |
Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast hansenula polymorpha |
| abstract |
Recombinant genetic constructs and strains of H. polymorpha having significantly increased ethanol productivity with a simultaneous decreased production of xylitol during high- temperature xylose fermentation are disclosed. The constructs include a H. polymorpha XYLl gene encoding xylose reductase mutated to decrease affinity of the enzyme toward NADPΗ. The modified version of XYLl gene under control of a strong constitutive HpGAP promoter was overexpressed in a Δxyll background. A recombinant H. polymorpha strain overexpressing the mutated enzyme together with native xylitol dehydrogenase and xylulokinase in the Δxyll background was also constructed. Xylose consumption, ethanol and xylitol production by the constructed strain were evaluated during high-temperature xylose fermentation (48° C). A significant increase in ethanol productivity (up to 7.4 times) was shown in the recombinant strain as compared with the wild type strain. Moreover, the xylitol production by the recombinant strain was reduced considerably: 0.9 mgχ(Lχh) -1 versus 4.2 mgχ(Lxh) -1 by the wild type strain. |
| priorityDate |
2008-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |