| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_baa7578a0aafb25785ccaa6f535244e9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d3e7af71949a19a74943f0bb13899c3e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf2a3a767ae32dfcda1f4e6642aeadc9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca243488748f843cc79cfe9b06564521
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab12a98ed4b5369f4ca0d19a05e11992 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2009-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca5acd750e85265086aa442ac4f8e0f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcae602dae06b47c741d04117eb7cad6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfc80e1c61bb9ed3a01d9abe57849bc6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c82ab90a492678c5526163513849ee29 |
| publicationDate |
2009-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009141141-A1 |
| titleOfInvention |
Method for high throughput peptide/protein assay generation and assays generated therewith |
| abstract |
The invention relates to a method for the determination of an MRM or SRM assay for a protein of interest, a peptide of interest, or a group of proteins/peptides of interest or a whole proteome. It essentially includes the following steps: (1) a list of proteins of interest is selected and for each member at least one or a list of candidate proteotypic peptides is derived (2) this at least one peptide is synthesized/generated essentially without subsequent purification; (3) this at least one unpurified peptide is analyzed by selected reaction monitoring (SRM) preferably coupled to liquid chromatography (LC-SRM) or analogous techniques; (4) validation and/or optimisation of the corresponding assay of the at least one peptide with determination of the SRM coordinates for a peptide/protein of interest and/or of a regulator of interest is achieved. A protein sample of interest is enzymatically digested and can then be analyzed in SRM mode or time-constrained SRM mode, using elution times to trigger acquisition of the set of selected SRM traces, thus drastically increasing the throughput. The analysis allows to detect and quantify the set of peptides/proteins of interest. The method additionally relates to a tagging strategy to achieve absolute quantification of the peptides/proteins of interest at low-budget and high-throughput. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3062099-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2015132901-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2012205337-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2659267-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9506167-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10215761-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105659077-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105659077-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012197258-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2011352231-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9551719-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2663863-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10302656-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10119975-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9372195-B2 |
| priorityDate |
2008-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |