| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_175b5214852f0665a92ba186e260dfbc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee8998357f4852ba9f5aa18b0fabd434
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41635fbe33bae592e34171b46c6e8003 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate |
2008-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c3091be62124b1982879c3608c5db41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35e457fd1451774c74753a2227b92913 |
| publicationDate |
2009-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009140795-A1 |
| titleOfInvention |
A method and kit for purification of recombinant proteins using a self-cleaving ptotein intein |
| abstract |
Provide is a method for purification of recombinant proteins using a self-cleaving protein intein, which comprises the steps of: recombinantly expressing a fusion protein which comprises a target protein domain, a self-cleaving intein and one or more domains for binding hydrophobic granules in host cells, wherein the self-cleaving intein is located between the target protein domain and one or more domains for binding hydrophobic granules; making the fusion protein releasing from host cells, then adding hydrophobic granules to it and incubating them; collecting hydrophobic granules after incubation and adding lysis buffer to allow the self-cleaving intein of the fusion protein to self-cleave; separating and removing the hydrophobic granules to obtain basically purified the target protein solution. A kit useful for the method is also provided. |
| priorityDate |
2008-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |