http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009139919-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65f43c3698fa731a1d7150bdd44a2732 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de094784025e53546f898a3de5a5e393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_11c5298388bf5090410400c6e8f51ed1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76f848d9755383c1f9148a3ccefb776d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2009-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_054973ebef1b1d22e2b6c913b8b8edb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e82d07aef45ad01953f872ca514011be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01fd91e8592384ade930351c9358be37 |
| publicationDate | 2010-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2009139919-A3 |
| titleOfInvention | Methods for diagnosis of clostridium diffcile and methods and vectors for recombinant toxin expression |
| abstract | Cell-based methods for rapid real time assay of a presence of Clostridium difficile toxin and/or cells are provided, using an assay having a toxin-enhancing antibody and a sensitive cell line carrying FcyR receptors, and kits for this assay. An ultrasensitive cell based immunocytotoxicity assay for detecting less then 1 pg/ml of C. difficile toxins in clinical samples. A TcdA-specific monoclonal antibody, AIH3, was found to significantly enhance the cytotoxicity of TcdA to macrophages and monocytes. The AIH3-dependent enhancement of glucosyltransferase activity, cytoskeleton disruption, and TNF-a production induced by TcdA was demonstrated also in RAW 264.7 cells. Methods for high level recombinant expression of C. difficile toxins in Bacillus cells, and vectors for expression, strains of Bacillus carrying the vectors are provided. |
| priorityDate | 2008-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.