http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009137574-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a036559e133cf9145349f432c9caee3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2efbe95965e682e179dfd266c9980505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4032d4610f4b8faa658596427ae7243a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_658da3351c95cae75f955712944c5e22 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-242 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2414 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01008 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2009-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8268f6739c6421f3b767c45ef42c99a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04df2d65bc144c91249e713f18176ae2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cfff44a9ebb9ed3c618844a18317d8b |
| publicationDate | 2010-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2009137574-A3 |
| titleOfInvention | Development of strains of the thermotolerant yeast hansenula polymorpha capable of alcoholic fermentation of starch and xylan by expression of starch and xylan degrading enzymes |
| abstract | Genes SWA2 and GAMl from the yeast, Schwanniomyces occidentalis , encoding α- amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha . The expression was achieved by integration of the SWA2 and GAM l genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha - glyceraldehyde-3 -phosphate dehydrogenase gene ( HpGAP . Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee , and a xlnD gene coding for β-xylosidase was obtained from the fungus Aspergillus niger . Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production. |
| priorityDate | 2008-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 234.