| abstract |
The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor. |