http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009105830-A1

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d5456aaa93c4ef62da705ea9aa92dff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ce5693eb54895786af89cd2128c151c
publicationDate 2009-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2009105830-A1
titleOfInvention Analysing methylation specific pcr by amplicon melting
abstract The present invention provides a method of evaluating DNA methylation in a sample. The method comprises (i) reacting the DNA with an agent that differentially modifies methylated cytosine and non-methylated cytosine to produce modified DNA, (ii) amplifying the modified DNA by methylation specific PCR to produce amplified DNA, and (iii) subjecting the amplified DNA to melting analysis. In the method the methylation specific primers are selected such that the sequence between the primers includes a region of known sequence variation and/or at least one cytosine nucleotide.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022002958-A1
priorityDate 2008-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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