| abstract |
The present invention relates to a growth medium for in vitro stem cell expansion comprising: Selenium 5ng/ml to 0.1mg/mL, Transferrin 5mg/ml to 100mg/mL, Insulin 2.5μg/ml to 1mg/mL, Pyruvate, preferably sodium pyruvate, 0.05 to 1 mM, L-glutamine 0.5 to 10 mM, Nucleosides 1 to 100 μg/ml at least one amino acid, preferably non-essential amino acid 5 to 1000 μg/ml, Iscove's modified Dulbecco ' s medium (IMDM) up to 1L. |