| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88b2ef92bb900789a7236d04e7528918
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_262a6183634e7d2b1341c174ced796ae
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2f0eb95a29376014855fecde239cc1b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_173bc2c63b34988699010ba05e5b1376
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc5b2bbba19e854ff3db421b60f8e6c3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-55 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N37-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8286 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-50 |
| filingDate |
2008-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0c840af04d37599c9b66770e863f4c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb188ab2ba8701241f7ac203313222b5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f69b4f6d1431684cead33919f4476b3b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c340b5b507b3ec3d6067dadbff04e46 |
| publicationDate |
2009-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009076426-A1 |
| titleOfInvention |
Engineering zymogen for conditional toxicity |
| abstract |
The ADP-ribosyltransferase, Vip2, exerts its intracellular toxicity in insects by modifying actin and preventing actin polymerization. Due to the nature of this toxin, expression of Vip2 in planta is lethal to the plant. Described herein are methods of making zymogens of toxic proteins that are benign in a non-target organism and are activated in a target organism. Disclosed herein are methods of engineering a random propeptide library at a terminus of a toxic protein and selecting for malfunctional variants in yeast. Using this method a selected proenzyme possesses reduced enzymatic activity as compared to the wild-type Vip2 protein, but remains a potent toxin towards corn rootworm larvae. The Vip2 zymogen can be proteolytically activated by corn rootworm digestive proteases. |
| priorityDate |
2007-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |