| abstract |
The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme in the relative absence of serine protease enzyme contaminants. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising Bacillus strains engineered to be deficient in multiple serine proteases, and their use in production of recombinant neutral metalloprotease(s). |