http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009057931-A3
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78d6e4f7cab73de0c2252d6e3ab89cf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd38569bbb4e417282d32a41444fddf9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c58072f9a5d00839faab50b0384585a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_afa04922d2247f6cc4458fad8791a33f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd15fea28fa5c3272dd3e41dcf3b0dd3 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
filingDate | 2008-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5edb665a0fc774bde5086a25e00793fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7378052a49d81e9f0383f8cf917e4c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0f1f59fa28582cae240b04c3d551a9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51b7f13f655a74b0a488a6b84c3b1699 |
publicationDate | 2009-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2009057931-A3 |
titleOfInvention | Dried composition for hot-start pcr with long-term stability |
abstract | The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long- term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR. |
priorityDate | 2007-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.