http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009057931-A3

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filingDate 2008-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5edb665a0fc774bde5086a25e00793fe
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publicationDate 2009-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2009057931-A3
titleOfInvention Dried composition for hot-start pcr with long-term stability
abstract The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long- term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.
priorityDate 2007-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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