http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009047778-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e0f7268aed9a3a71da2f8dc65a23b55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d606c5130fcfad0e1039ef1a24f53277 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e5b1405088717c4ea1ee0c7fde1675b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3a0d6297082cc4413878148c761e49b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate | 2008-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d267508e9d5f59f2868a8891215c1fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_262f33b4c31e798aee0253460ef04b5e |
| publicationDate | 2009-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2009047778-A1 |
| titleOfInvention | In vivo labeling of mammalian cone photoreceptors by intravitreal injection of fluorescently tagged peanut agglutinin |
| abstract | A method for specifically labeling outer and inner segments and pedicles of mammalian cone photoreceptors in vivo comprising Intravitreal injection of 2 μl for adult anesthestized mouse (C57BL/6 J), 10 μl for guinea pig (Duncan Hartley) of 20 μl for monkey (Macaca mulatta) of PNA conjugated with a fluorescent probe (Rhodamine or Fluorescein) at a concentration of 0.005%, 0.01%, 0.02%, 0.05% or 0.5% (only 0.5% for guinea pig and monkey) with a glass electrode with tip diameter of ~ 1 μm or an insulin syringe posterior to the limbus into the vitreous of the eye, Removing the eye under anesthesia at various time points (mouse: 0.5, 1, 2, 4 and 8 hours; 1, 2, 4, 7 and 10 days; 1 and 3 months; guinea pig and monkey: 30 minutes) after the PNA injection, Hemisecting and fixing the posterior eye-cup in 4% paraformaldehyde for 1 hour at 4°C, Flat mounting the retina on a glass slide or cryosectioning, and observing PNA labeling with a fluorescence microscope. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012041305-A1 |
| priorityDate | 2007-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 132.