| abstract |
The present invention provides systems for measuring protein translation and methods for measuring overall translation activity in viable cells or subcellular compartments. The methods of the present invention identify general ribosomal activity, if desired at sub-cellular resolution, thereby providing a signal indicating the rate of any of the steps of protein synthesis selected from initiation, elongation, termination or recycling. The translation system of the present invention can be used to identify translation modulators in high-throughput-screening (HTS). |