| abstract |
Methods of screening for modulators of Racl, RhoA or focal adhesion kinase (FAK) in endothelial cells are disclosed. The effect is determined by- measuring endothelial permeability, Racl or RhoA activity and phosphorylation of FAK. Vascular permeability may be stimulated by thrombin. Method of screening for compounds that prevent vascular leak in a warm-blooded animal undergoing systematic inflammatory response triggered by LPS, using fluorescence labelled micro-beads. A method of screening comprising a combination of all said methods is also disclosed. Compounds and pharmaceutical preparations being identified by said methods. |