| abstract |
The present invention provides compositions, kits and methods for selective enrichment, isolation, purification, production, tagging, cloning, amplification, detection, quantification, characterization, and assay of nucleic acid molecules that either have a monophosphate group, a polyphosphate group, or a cap nucleotide on their 5' terminus. The resulting 5'-monophosphorylated RNA molecules generated using the method are 5' ligation tagged by ligating an RNA acceptor oligonucleotide to their 5' ends using RNA ligase. The tagged RNA can be used for full-length-selected synthesis of first-stand cDNA, or double-stranded cDNA for a variety of uses and applications. |