| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f82f52b759551b11040f653b3af72386
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed7919c5019f9a037e4036b8ee0dbe10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2008-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b8e1fda2e133feaf6f94dc0fe9555d3 |
| publicationDate |
2009-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009004335-A1 |
| titleOfInvention |
High multiplex nucleic acid amplification |
| abstract |
This invention relates to the amplification of multiple nucleic acid target sequences. Forward and reverse compound primers comprising a common amplification sequence and a target-specific primer sequence are immobilised at a site on a solid support. Target regions of single-stranded template DNA are primed and copied by the forward primer to produce a first extension product. The template DNA is removed and the first extension product is primed and copied by the reverse primer to produce a second extension product. The second extension product has common amplification sequences at each end and is bulk-amplified in solution by regular PCR employing primers that target the common amplification sequences. These methods allow highly multiplexed amplifications to be performed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011056133-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10501786-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012157322-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9624489-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108603224-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9309556-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10072283-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9334529-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017106777-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108603224-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021194640-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11117113-B2 |
| priorityDate |
2007-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |