| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5d2d9fcd7b43180522a8bc4063ecb52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cfc868200932167b823669075d8dd40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb13873f6bc564d17413c08320c71c1f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2008-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65b2cc0f376ac44b2ff893f3530551ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_839e58f76d5b8a61da150ae9eeb87e9b |
| publicationDate |
2008-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2008157316-A1 |
| titleOfInvention |
Improved method for rna isolation |
| abstract |
The present invention provides methods for isolating RNA from a biological sample, preferably an RNAse-rich biological sample. The method includes mixing a biological sample with an extraction reagent to form a homogenate, extracting the RNA in the homogenate with an organic solvent, and precipitating RNA from the aqueous phase. In some aspects the biological sample and the extraction reagent are combined at a ratio of less than 50 mg of the biological sample to 1 ml extraction reagent, preferably, no greater than 25 mg of the biological sample to 1 ml extraction reagent. In some aspects, the organic solvent includes phenol and chloroform in a ratio of between 4 parts phenol to 1 part chloroform (4: 1) and 6 parts phenol to 1 part chloroform (6: 1). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103068979-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10647978-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5906740-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2607482-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2607482-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101641113-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20130098163-A |
| priorityDate |
2007-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |