| abstract |
Method for determining the content of a chromophoric and nonfluorescent compound, referred to as first compound, of a biological tissue (30) of a biological entity, wherein said biological tissue (30) also comprises a chromophoric and fluorescent compound, referred to as second compound, and wherein said method comprises at least one iteration of the following operations: emission, in the direction of said tissue (30), of a first, “reference”, optical radiation and of a second, “measuring”, optical radiation, each chosen so as to induce a fluorescence radiation from said second compound, wherein each of said first and second radiations is partially absorbed by said first compound, measurement of said fluorescence radiations induced by said first and second radiations, and determination, from said measurement, of the content of said first compound in said tissue; characterized in that said method also comprises at least one compensation for saturation of the measurement due to too great an absorption of the measuring radiation by said first compound, wherein said compensation comprises a choice, for said measuring radiation, of a wavelength corresponding to a weaker absorption in the absorption spectrum of the first compound. |