http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008141795-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4db91b9bbeb41c9675bf10b8e6cbcb9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e54adfb5ff0b4710aba959d251abb8a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf2a3a767ae32dfcda1f4e6642aeadc9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2008-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e6f7a46a0a52daab0f9c2fa93ff0bd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec828ed7f84e130cb8a78810c4e188bf |
| publicationDate | 2008-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2008141795-A2 |
| titleOfInvention | Cell-free protein synthesis of deuterated proteins for structural and functional studies by nmr spectroscopy |
| abstract | A method for cell-free synthesis of at least partially deuterated proteins, peptides or assemblies thereof, in particular for NMR studies, is described. The method comprises at least the following steps: a) preparation of a cell extract under essentially non-deuterated conditions; b) exchanging D2O into the cell extract in a buffer exchange process to form a deuterated cell extract; c) cell-free synthesis using at least the deuterated cell extract, expressed and purified RNA polymerase, at least partially deuterated amino acids and a plasimd or a linear template DNA encoding said protein or peptide; d) isolation and purification of the protein or peptide. Furthermore an extract for use in such a cell-free synthesis and a kit for use in such a cell-free protein synthesis is described |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108169272-A |
| priorityDate | 2007-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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