http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008132755-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4d2d4ee042ea8d219d0c8376799f70a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_16cf2434af92c8f9ffe41cfbee175d45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7bc1a1eb5730a9db0348ae8b0fa0dac9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5094 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2007-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9361d1cec4ec536b515c4c220d2ff6d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43a0bb04378f53eaa44a23f964e22e6b |
| publicationDate | 2008-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2008132755-A1 |
| titleOfInvention | A test kit for estimating cd4 + /cd8 + t-cells in a human blood sample |
| abstract | A test kit for estimating CD4 + / CD 8 + T-cells in a human blood sample. It comprises at least one microscopic glass slide provided with at least four spots; saline wash buffer; reverse osmosis water; poly-L-Lysine solution; phosphate buffered saline; histopaque; RBC Lysis buffer; a carbonyl iron powder; antibody diluting fluid; glycine buffer; gluteraldehyde; primary antibody selected from Anti-CD4 monoclonal antibody, Anti-CD8 monoclonal antibody and Anti-CD5 monoclonal antibody; secondary antibody selected from Rabbit Anti-Mouse antibody and Swine Anti- Rabbit antibody; enzyme linked conjugate like Peroxidase Anti-Peroxidase antibody (PAP); Chromogen; substrate buffer; slide holding jar; haematoxylin; a mounting solution and a cover slip for the slide. Also a method of estimation of CD4 + / CD 8 + T-cells in a human blood sample by using the test kit comprising immobilizing T-cells isolated from the blood sample on all the spots of a microscopic slide provided with at least four spots and pre-treated with poly-L-lysine followed by fixing the T-cells with solution of glutaraldehyde. The T-cells are neutralized with glycine buffer; antibody diluting fluid is added onto the T-cells followed by primary monoclonal antibodies and secondary antibodies and finally enzyme linked conjugate. The T-cells are dyed with chromogen, stained with the haematoxylin. The stained and unstained T-cells on all the spots of the slide are counted under a microscope. Also use of the test kit for the CD4 + and CD8 + T-cells estimation. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014080017-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114196627-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10928405-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9063127-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112964877-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113504092-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9709556-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112964877-B |
| priorityDate | 2007-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.