| abstract |
An Apocrine cell line An apocrine cell line is obtained by the steps of isolating an apocrine cell from primary tissue, culturing the isolated cell in a first culture medium, removing unattached cells from the first culture medium and transferring said unattached cells to a second culture medium comprising an effective concentration of a phorbol ester, and thereby establishing an apocrine cell line exhibiting long-term proliferation capability, which after many cultures is indicative of indefinite proliferation. |