http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008109161-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b53b0cc714e4bd626ab114ff1b98286b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_25535dceb0e40948bdbe375134c28121 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ef416fe4f66ebb1021cecfb0ba5ae45d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fce8f9093a72aa10655f5133eab77cef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2008-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da416d8c40d0643ff7efa8fc6d080d7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75b61160248400472e02b8610ead0563 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52449614ecc4a4b6bc1969c61f9f341f |
| publicationDate | 2008-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2008109161-A2 |
| titleOfInvention | Deeply quenched enzyme sensors |
| abstract | Sensors for detecting enzyme activity are provided that include a substrate module comprising a substrate for the enzyme of interest and a fluorescent label, a quencher, and a detection module. The detection module binds to the substrate module either before or after the enzyme acts on the substrate and sequesters the label from the quencher, resulting in an increased signal from the label. Sensors for detecting enzyme activity are also provided that include a substrate for the enzyme, a label, and a quencher that quenches the label. Action of the enzyme on the substrate results in a conformational change that relieves quenching. Sensors for detecting protein-protein interactions are also provided that include a quencher and a labeled first polypeptide. Binding of the first polypeptide to a second polypeptide sequesters the label from the quencher, resulting in an increased signal from the label. Methods using the sensors to detect enzyme activity and to screen for compounds affecting enzyme activity or to detect protein-protein interactions and to screen for compounds affecting protein-protein interactions, respectively, are also described. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012016704-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020214573-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014057044-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012128722-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102883773-A |
| priorityDate | 2007-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1536.