| abstract |
The present invention relates to a mutant hydroxylase with increased activity and greater substrate specificity for phenylacetyl-7-ADCA for the production of phenylacetyl deacetyl-7- ACA, which carries one or more amino acid modification at residue positions when compared with the wild type hydroxylase from the following group of residues, Glutamic acid at position 16, Tyrosine at position 38, Proline at position 72, Threonine at position 90, Valine at position 150, Proline at position 186, Valine at position 221.Methionine at position 229, Threonine at position 273, Threonine at position 304 and Alanine at position 311. |