http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008097803-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_477f61b856ccc7beb7e9ddabcc42d734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca72b13a611dddee13d974b2af42e522 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1ca8afbf0adbecbb9dfbf087e9f0312a |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D57-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-447 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D57-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
filingDate | 2008-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9d8b1b4b29855600bd98e9855299beb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f82962400eaa2a37288039c562f4892 |
publicationDate | 2008-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2008097803-A1 |
titleOfInvention | A rapid homogeneous immunoassay using electrophoresis |
abstract | The present invention concerns methods, compositions and apparatus for detection and/or determination of concentration of target molecules. In preferred embodiments the methods comprise allowing target molecules to form a complex with a first binding agent conjugated to a detection molecule and a second binding agent conjugated to a capture molecule, allowing the complex to further bind to an essentially uncharged polymer capable of binding to the capture agent, and performing a vertical gradient electrophoresis to separate the complex from unbound target, first and second binding agents and polymer. The intact complex is concentrated by electrophoresis at a stacking layer and the detection molecule is detected and/or quantified. Because the complex contains a very high mass to charge ratio, it becomes essentially immobile at the stacking layer, while unbound components migrate through the stacking layer and are separated from the complex. This provides a very rapid and sensitive assay that can detect very low concentrations of target molecules in short time. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11255815-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021211904-A1 |
priorityDate | 2007-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.