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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
filingDate 2008-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbb38a84d352aa70b25fcb1709813d6e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_25cf9009b3604389f282887e3d346390
publicationDate 2008-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2008095501-A1
titleOfInvention Horizontal antigen retrieval
abstract The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100°C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.
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priorityDate 2007-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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