http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008094273-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a47d7c7bfa7846abc01c244bab912a74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_86775abbb2e3cd5e4150871f347c82c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_957bcc825a77326a549f7f1a6cdccff6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b414516393cfbe054ed4afadee42776b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d6f7da1e425c8a3ef1da21e08f4c196 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6fe38a6a1358c1467f7a4ee80a9c347b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_997921e22e301bd3ad3a6276b9377d65 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5432 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
| filingDate | 2007-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f65002758ef76ce1be9b90ebcf20772f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dabad99e044401922650ffb07ea0791f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_743bf0b72ebd4e0477ec0ddeed9762cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b1a46005a264920e43ce10827e036ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41d79abd510c8b4e7ad58c95e2f981d1 |
| publicationDate | 2008-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2008094273-A3 |
| titleOfInvention | Detection of analytes in samples using liposome-amplified luminescence and magnetic separation |
| abstract | The invention relates to the encapsulation of luminescence-related molecules, including but not limited to, adenosine triphosphate (ATP), adenylate kinase (AK), alkaline phosphatase (ALP), luminol and luciferin/luciferase cocktails, within liposomes. These liposomes can be employed to enhance the luminescence detection of microorganisms and compounds in various products and samples. The liposomes containing the luminescence-related molecules can bear a probe which has a specific sequence or structure that, in turn can be used to hybridize to, or couple with, a portion of the target analyte. Within the same assay, paramagnetic beads can bear a probe having a specific sequence or structure that, can hybridize to, or couple with, a second portion of the target analyte to create a complex of analyte bound to paramagnetic beads and liposomes. This type of assay can be often referred to as a 'sandwich' assay. Once the probes hybridize to, or couple with, their targets, a complex can be formed of the paramagnetic beads, the analyte, or portion thereof, and the liposomes. This complex can then be washed to remove those components that are non-hybridized or non-coupled. Then, the paramagnetic bead-analyte- liposome complexes can be isolated from the sample using magnetic separation techniques and can be treated so as to release their encapsulated ATP, AK or other luminescence-related compounds. The resulting luminescence can then be determined in a chemical assay. This determination can be qualitative (i.e., an absence/presence assay) or quantitative (i.e., which can measure a specific amount of analyte present). Through the use of a cocktail of probe types, the assay can also qualitatively or quantitatively measure the presence of more than one analyte simultaneously. This type of assay can be of commercial importance in clinical and forensic applications, the personal care, pharmaceutical, food and beverage markets, as well as in environmental sample assays. |
| priorityDate | 2007-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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