| abstract |
Provided is a long-term preservation method for a skin tissue and a dermal layer of a skin cell for transplantation, including: a first glycerol solution processing step of cleaning a skin tissue taken out of a skin tissue donor, immersing the skin tissue into a 45-55% glycerol solution at normal temperature, and performing stirring thereon; a second glycerol solution processing step of immersing and preserving the first glycerol solution processed skin tissue into a 65-75% glycerol solution at normal temperature! and a third glycerol solution processing step of immersing the second glycerol solution processed skin tissue into an 80-90% glycerol solution at normal temperature and continuously preserving the skin tissue at a temperature of 4°C to stabi lize the skin tissue. Therefore, any state of dehydrated skin tissue or an acellular dermal layer for transplantation preserved in the aforementioned method contains the glycerol solution instead of water. Accordingly, shapes and functions of various elements such as protein/collagen used to re-construct the skin for transplantation can be maintained, and a basement membrane for correcting re-attachment of a living endothelial cell or epithelial cell can be provided safely. In addition, a structure of an original tissue can be maintained without structural changes and damages. |