| abstract |
Certain aspects of the present invention provide informed methods for designing and engineering protein variants and identifying novel functional polypeptides, including novel therapeutic products. Aspects of the invention include preparing libraries of nucleic acid variants that are based on rearrangements of predetermined fragments from an initial gene sequence. The libraries contain a high percentage nucleic acids that express functional polypeptide variants and/or variants that have at least one predetermined property of interest. The expressed polypeptide variants are evaluated to identify novel products. Aspects of the invention can be used to identify novel classes of polypeptide variants, for example novel classes of therapeutic products. Aspects of the invention also provide medical, pharmaceutical, industrial, agricultural, environmental, and other uses for protein variants. |