| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7e4045aed6f9e7263c3e6ac95484686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_360cfdb96e32c2b3db1c33070aee7b80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a2352f78bd285780ad3e2a066ed4f10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa769316abd1096f6548514f14f70a88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95eaed79dec59391549e8016815b3a8f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate |
2007-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4df6c331a2da048bbb9bebd5d756ea8b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_418e6d7828121f961fb96fe51d223355
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62d0b1bdcba41c6a62cd17f2a31c81ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57ba120692dda1ea81ef1eda4584d3d8 |
| publicationDate |
2008-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2008072717-A1 |
| titleOfInvention |
Method of evaluating skin barrier function, method of screening material for enhancing skin barrier function using the evaluating method, the material for enhancing skin barrier function, and cosmetic containing the material for enhancing skin barrier function |
| abstract |
An object of the present invention is to provide a technology for developing an excellent material for enhancing skin barrier function and to provide a cosmetic which is developed based on the technology and has excellent ability to enhance the skin barrier function. The present invention relates to a method of evaluating skin barrier function, comprising: 1) culturing normal human epidermal cells in wells with a semipermeable membrane bottom; 2) adding a medium not containing metal ions to be measured to the above of cells in each well and a medium containing the metal ions to be measured to the below of the cells in each well, provided that the below is a side where the cells are adhered to the semipermeable membrane, and the above is an opposite side thereto, and culturing the cells under metal ion loaded condition; 3) measuring a concentration of the metal ions in each medium; and 4) calculating permeability of the metal ions in an epidermal cell layer based on the measured concentrations of the media to evaluate the skin barrier function. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013100355-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013100356-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109152724-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109152724-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011162476-A |
| priorityDate |
2006-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |