| abstract |
The present invention provides oligonucleotides complementary to a target polynucleotide, the oligonucleotide comprising an LNA unit, a mismatch nucleobase, and an allele-specific nucleobase corresponding to an allele of the target polynucleotide. The invention further includes methods of using such oligonucleotides to detect and quantify the frequency of particular alleles of a target polynucleotide. |