| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03f176aed1a2dc6df5e93bc23d02a11b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_144f594513aec7b42679f432e92531e8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4c41a0351aba1f52d0d886aeb5dce20f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2007-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa2b3cf35992fe6579a27bb040afc57f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75451ac7dfb1dfaacf48e6bbd2dfa3d3 |
| publicationDate |
2008-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2008039664-A1 |
| titleOfInvention |
Nucleic acid purification method using anion exchange |
| abstract |
The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes adding in the elution solution a composition such that the pH of the aqueous mobile phase is between about pH 9 and about pH 13, wherein the presence of the composition in the elution solution provides an increase in nucleic acid recovery, as compared with the recovery in the absence of the composition. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2664917-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9447460-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9481881-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103348242-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2664917-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2012500651-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103348242-B |
| priorityDate |
2006-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |