| abstract |
The present invention relates to a method of preparing a soft tissue filler composition for injection, which comprises the steps of: 1) digesting an autologous dermal tissue isolated from the patient's own skin and separating into single cells; 2) culturing and proliferating the isolated dermal cells via in vitro serum-free cultivation to obtain an autologous dermis-derived cell culture material containing dermal fibroblast stem cells, dermal fibroblast transit amplifying cells, dermal fibroblasts and collagen; 3) centrifuging the autologous dermis-derived cell culture material to separate an autologous dermis-derived cell pellet; and 4) suspending the autologous dermis-derived cell pellet in a glucose injection or a random injection to obtain a suspension for injection; and a soft tissue filler composition for injection prepared by the same. |