Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_160f7d605ac4e35e262dfdae560e74db http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_916d65c89256dd77853acee46349ef9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e6516a0437dd8e0974bede4dcc89165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9bceffddb90b9b3da8a772212961764b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ea7cc5d28840608f523eab9fa9d7f95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b89b501d14f29893183f242a4bf37533 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2006-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b03d12f9228a7e5d77da7bf6c0c6a9a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_451247486d5536b7c9875792b169ea84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c455a4f5ad780013eef52dc38885b0a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b609206981732d1bc0e39635b45777e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc836e4765b8ec88b0ce20889525a6df |
publicationDate |
2007-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2007135760-A1 |
titleOfInvention |
METHOD FOR SYNTHESIZING NUCLEIC ACID USING DNA POLYMERASE β AND SINGLE MOLECULE SEQUENCING METHOD |
abstract |
A method for synthesizing a nucleic acid capable of carrying out an extension reaction continuously and a single molecule sequencing method capable of acquiring base information accurately at a high speed are provided. The method for synthesizing a nucleic acid in which a complex of a target nucleic acid to which a primer oligonucleotide is hybridized and DNA polymerase β is formed, a fluorescently labeled dNTP is allowed to be incorporated into the DNA polymerase β, the fluorescently labeled dNTP is bound to the 3' end of the primer oligonucleotide and the fluorescently labeled dNTP is allowed to be incorporated into the DNA polymerase β continuously, whereby a nucleic acid complementary to the target nucleic acid is extended from the 3' end of the bound fluorescently labeled dNTP. The method for sequencing a single molecule of nucleic acid including a step of extending a nucleic acid complementary to a target nucleic acid by the method for synthesizing a nucleic acid and performing sequencing of the target nucleic acid by detecting fluorescence of the fluorescently labeled dNTP incorporated into the DNA polymerase β sequentially. |
priorityDate |
2006-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |