http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007129778-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14430ce0c28e36044927f5834e4d02ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4e745d2242b8e3b43ca8f2d4cc7291ed |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8900a6f2d375201a870b77c2635987a7 |
| publicationDate | 2007-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2007129778-A1 |
| titleOfInvention | Method for amplifying unknown dna sequence adjacent to known sequence |
| abstract | The present invention relates to a method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, which comprises the step of (a) performing a primary amplification of the unknown nucleotide sequence using a DNA walking annealing control primer (DW-ACP) and a first target specific primer (TSP) hybridizable with a site on the known nucleotide sequence; in which the step (a) comprises: (a-1) performing a first-stage amplification of the unknown nucleotide sequence at a first annealing temperature, using a first degenerate DW-ACP containing (i) a degenerate random nucleotide sequence to hybridize with the unknown nucleotide sequence and (ii) a hybridizing nucleotide sequence substantially complementary to a site on the unknown nucleotide sequence, wherein the first annealing temperature enables the first degenerate DW-ACP to function as a primer, whereby a first degenerate DW-ACP extension product is generated; and (a-2) performing a second-stage amplification of the amplification product generated from step (a-1) at a second annealing temperature which is higher than the first annealing temperature, using the first degenerate DW-ACP as used in the step (a-1) and the first TSP, under conditions in which each primer anneals the its target nucleotide sequence, whereby a primary amplification product is generated. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3597657-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11781179-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103397029-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103397028-A |
| priorityDate | 2006-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 249.