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filingDate 2007-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4040afe412a1c24e1a4685abe65f21b
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publicationDate 2007-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2007102400-A1
titleOfInvention Apparatus for real-time detection of nucleic acid amplification product
abstract It is intended to provide an apparatus for the real-time detection of a nucleic acid amplification product by which error-causing factors on the apparatus can be effectively eliminated or lessened without employing a second fluorescent signal to be used for the correction. A temperature cycle is applied to a plural number of wells (7A) and the fluorescent intensity from a nucleic acid amplification product in each well (7A) is detected in real-time. The measured fluorescence [DNA]raw obtained from the well (7A) and the measured fluorescence [DNA]bg obtained from the connecting wall in the neighborhood of the well (7A) are detected. By subtracting the measured fluorescence [DNA]bg from the measured fluorescence [DNA]raw, the fluorescence [DNA]real of the well (7A) is determined.
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