| abstract |
An integrated polynucleotide amplification and nucleotide sequence identification system comprises an expendable microfluidic cartridge and a reusable external unit. Sample fluids and reagents are confined to the expendable microfluidic cartridge for processing. The reusable external unit comprises pumps, valves, sensors, and temperature controllers. Polynucleotide sample amplification through polymerase chain reaction (PCR), and nucleotide sequence identification using oligonucleotide arrays can be conducted in an automatic, integrated process. |