| abstract |
It is intended to provide an immunochromatographic detection device which detects PBP2’ specifically produced by a multidrug-resistant Staphylococcus bacterium by an immunochromatographic detection method sensitively, simply and rapidly and is capable of determining infection with a multidrug-resistant Staphylococcus, a diagnostic method using the detection device, and a diagnostic kit including the detection device. The invention is directed to the immunochromatographic detection device for detecting a multidrug-resistant Staphylococcus which includes a sample supply unit which supplies a sample solution which is assumed to contain a multidrug-resistant Staphylococcus or a solution which is assumed to contain PBP2’ released from a cell wall by a pretreatment of a sample onto a sheet-shaped solid-phase support, a labeling reagent unit which retains a labeling reagent in which an antibody specifically binding to PBP2’ is labeled spreadable on the solid-phase support, and a trapping reagent unit in which a trapping reagent capable of trapping a complex of PBP2’ and the labeling reagent by specifically binding to the complex is immobilized, and in which an amphoteric surfactant, an anionic surfactant and/or a nonionic surfactant is contained in the trapping reagent unit or in the solid-phase support upstream of the trapping reagent unit. |