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filingDate 2006-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2007-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2007067732-A3
titleOfInvention A scintillation proximity assay for identifying inhibitors of fatty acid amide hydrolase
abstract Assays for screening compounds suspected of binding fatty acid amide hydrolase (FAAH) using scintillation proximity assay (SPA) technology are described. The assays utilize the interactions of reversible inhibitors such as [3H]-R(+)-methanandamide (MAEA) and membrane-associated FAAH5 such as FAAH- containing microsomes, to evaluate the displacement activity of candidate FAAH inhibitors. The assays are specific for FAAH where FAAH binding compounds can be detected. Various embodiments of the assay have been validated and demonstrated to be simple, sensitive and amenable to high-throughput screening. Kits for performing the assays are also disclosed.
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