| abstract |
The present disclosure provides methods of increasing the activity of radical S-adenosyl methionine (SAM) enzymes, for example by increasing expression of ferredoxin, flavodoxin, flavodoxin reductase, or combinations thereof in a cell expressing a radical SAM enzyme. Such methods can be used to increase expression of a radical SAM enzyme product, or a downstream organic chemical. Also provided by the present disclosure are cells that have increased expression of ferredoxin, flavodoxin, flavodoxin reductase, or combination thereof. Such cells can be used to practice the disclosed methods. |