| abstract |
Microfluidic devices are disclosed for carrying out cyclic or iterated reactions such as PCR, LDR, and other cyclic or iterated reactions. A microchannel forms a closed loop, through which a reaction mixture may be thermally cycled an arbitrary number of times. Flow is preferably mediated primarily by electrokinetics. Multiple temperature zones may be employed along the course of a single microchannel loop, for example for PCR. Embodiments may be compact, automated, fast, and operable in continuous-flow mode. Real-time reaction monitoring may optionally be used. |