http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007043494-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e5482e4b7c2e0ccc62ea88d2b3aa054 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50bb20523039826c0b5b9d411abfabfd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_440b3059c9bcd1d85fd383f9b2ae1b40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4c96b78b4058104fe8a1e7f823a5fd9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2006-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_afd397a8dbc7a6d8ee3b1aa62f0be665 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b36bc20fdb5e17bc4bf3dd2637140afa |
| publicationDate | 2007-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2007043494-A1 |
| titleOfInvention | Method of collecting dna from biological sample |
| abstract | A method of collecting a DNA from a precious biological sample without impurity mixing efficiently in a simple and easy manner. There is provided a method of collecting a DNA, comprising sequentially performing: (1) the pretreatment step including the operation of fragmentizing of a biological sample of hard tissue used in whole or after cutting or crushing, and the subsequent decalcification operation of adding EDTA thereto; (2) the dissolution step of adding a buffer solution of solubilizing agent for dissolution of any DNA-protein complex contained in the biological sample together with a protease to the mixture (1) to thereby prepare a DNA solution; (3) the extraction step of adding a saturated phenol buffer solution of tris(hydroxymethyl)aminomethane hydrochloride or an aqueous solution containing sodium iodide/isopropanol to the complex solution (2) to thereby carry out deproteinization and thereafter obtaining a DNA extract solution by fractionation; and (4) the purification step of passing the DNA extract solution (3) through a column to thereby attain DNA purification. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101302508-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018193502-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107513528-A |
| priorityDate | 2005-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.