| abstract |
An improved method for the production of oligosaccharides with desired molecular weights through enzymatic hydrolysis of starch, comprising blending starch with water to obtain a starch slurry, mixing the slurry with 0.01 % - 0.03% CaCI2 based on dried starch, adjusting the pH to 5-7, adding 0.03 - 0.08% of heat-resistant α-amylases based on dried starch, and hydrolysing the starch to produce the desired oligosaccharides, wherein the improvement consists in controlling ultra-low DE value during the enzymatic hydrolysis step. The controlled ultra-low DE value is obtained by adjusting the amount of α-amylases during jet liquefaction at a temperature of between 100 to 130°C, and holding the liquefied starch in a laminar-flow tank for 20 to 60 minutes. The amount of desired oligosaccharides having 3 to 5 glucose units in the resulting product is thus increased to 70 % before purification, while the content of glucose is reduced to about 10%. |