| abstract |
The present invention relates to a novel method for determining a receptor- ligand interaction which is suitable for ultra sensitive pharmacological receptor profiling on cells, e.g. single living cells based on the repetitive application of reversibly binding fluorescent ligands with fast association- dissociation kinetics. The method allows the determination of single receptor molecules and ligand binding cycles on single cells in microfluidic structures. Thus, the present method is important for high throughput screening of compounds, membrane receptors and tissues. Further, novel fluorescent derivatives of α-conotoxins, particularly of α-conotoxin Gl as described. |