http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006084695-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec0ec66d5b439b158d9f4cb6de10bde5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4caa77d6ee705e63ba9d4a406c6e5094 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8891d6975556d69943998368cd77025f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_099e1a6f2fa10f6c7758d20e68b99ef8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2820-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-003 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2006-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35b253c8766b0c0809863d099e6ee9a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef5f0eb405938b5ec80693435d5c8ce8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c242bb90bebddcc61ff864c41a2ed50 |
| publicationDate | 2006-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2006084695-A2 |
| titleOfInvention | Tetracycline-controlled insertion element for systematic mutagenesis |
| abstract | The present invention relates to an insertion element, wherein said insertion element is a recombinant nucleic acid molecule which comprises, in 5' ◊ 3' arrangement: (i) a first recombines binding site; (ii) a resistance gene, operatively linked to a promoter and, optionally, to transcription control sequences; (iii) a tetracycline-controlled promoter; and (iv) a second recombines binding site, wherein said tetracycline-controlled promoter is oriented to allow transcription in direction of the second recombines binding site. The present invention also relates to a method for the generation of a conditional knock-out mutant of a cell, comprising the steps of: (a) introducing a nucleic acid molecule comprising the insertion element of the present invention into a cell, wherein the insertion element is transfected into the cell concomitant with purified recombines; (b) integrating the insertion element into the cellular genome; and (c) selecting cells for resistance against a substrate for which the resistance gene of the insertion element confers resistance; wherein prior to or concomitant with step (a) an expression construct for a tetracycline-sensitive (poly)peptide is introduced into the cell, said (poly)peptide being capable of binding to the tetracycline-controlled promoter of the insertion element and of modulating its expression. Finally, the present invention relates to a cell containing the insertion element of the present invention, to a method for identifying genetic determinants of pathogen survival and to a method for the generation of a transgenic animal and plant. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9115363-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2683827-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE49583-E http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012119203-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2683827-A4 |
| priorityDate | 2005-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 735.