http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006084201-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_343ec34acfd78083ff252e89aebca4bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8cfe5c3632a346235bee4dc77a894503 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-00 |
| filingDate | 2006-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0031c0d16cb874598b6842fe53b46437 |
| publicationDate | 2006-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2006084201-A2 |
| titleOfInvention | Method of isolating, labeling and profiling small rna and whole-genome transcripts |
| abstract | Disclosed herein is a method of selectively labeling non-messenger RNA molecules by isolating total RNA from a tissue or cell, dissolving the isolated RNA, blocking 3' ends of the RNA and adding T4 RNA ligase and a labeled nucleic acid adaptor, with the result that the T4 RNA ligase ligates the adaptor only to RNA having a 5' phosphate group such as small RNAs. A method of labeling the 5' end of mRNA isolates total RNA from a tissue or cell, dissolves RNA in RNase-free water, removes a 5' cap structure from the mRNA using tobacco acid pyrophosphatase (TAP), removes the TAP, blocks the 3' end of the RNA molecules; and ligates an adaptor to the RNA by adding T4 RNA ligase and a labeled DNA or RNA adaptor. A method of amplifying and labeling non-capped RNAs and/or capped RNAs is useful in expression analysis of the whole-genome transcripts from cells and tissues. Sense and antisense transcripts are labeled from different experimental approaches. In another embodiment, there is disclosed a method of sequence selection and probe design. Probes are designed complementary to the labeled target RNA. For small RNAs, sequences are selected for detections of mature, counterparts (ig miR*), and precursors. In another embodiment, there is disclosed a method of expression profiling small RNA by separating labeled RNA from capped RNA, providing a microarray comprising a plurality of probes hybridizable to small RNA, incubating the labeled small RNA with the microarray, washing unhybridized RNA from the microarray and drying the microarray, staining hybridized RNA on the microarray; and scanning the labeled microarray to determine the identity and quantity of labeling to the various nu'RNA probe sites and thus providing an expression profile of small RNA. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2283132-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10563250-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11473132-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11274340-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008040355-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11208688-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250278-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3967768-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016149021-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008040355-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9963735-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10954553-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2283132-A2 |
| priorityDate | 2005-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 109.