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publicationDate 2006-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2006083911-A2
titleOfInvention Method and apparatus for phase contrast quadrature interferometric detection of an immunoassay
abstract A phase contrast quadrature interferometric method and apparatus for determining the presence or absence of a target analyte in a sample, comprising using a laser beam having a wavelength h and a waist w, to probe at least a portion of a substrate having a reflecting surface that has been exposed to the sample. The reflecting surface includes at least a first region having a layer of recognition molecules specific to the target analyte and a second region that does not include a layer of recognition molecules specific to the target analyte. The method further comprises measuring a time dependent intensity on a photodetector of a substantiall only first quadrature at one of a pair of quadrature angles, of a reflected diffraction signal of the probe beam while probing the first region and the second region.
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