Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f79749e86afce9444a364ab60ca3584 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d3916bffb4f2b358de0c7bb15287e99e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2fec3fe758c5f51f1a8082c3759fe8b3 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6441 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6445 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
filingDate |
2005-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61697d2b65a6c73f3639b91309a6a9d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4af2ec9ef94154917a1c16ce22b7676b |
publicationDate |
2006-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2006083382-A2 |
titleOfInvention |
Measuring forster resonance energy transfer with polarized and depolarized light |
abstract |
The present application provides improved methods for assessing Förster resonance energy transfer (FRET) using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules. Depolarization of fluorescence emission is observed only in the presence of energy transfer. A simple detection strategy is described for fluorescence microscopy using laser scanning and wide field techniques. FRET between linked and unlinked green fluorescent proteins (GFPs) in living cells can be monitored with a larger dynamic range than in other techniques. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2535709-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2535709-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102890155-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8647887-B2 |
priorityDate |
2004-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |