| abstract |
The present invention relates to a plant vacuole targeting sequence X1X2X3PX4 wherein X1 is a hydrophobic amino acid, X2 is a basic amino acid, X3 is a hydrophobic amino acid, P is proline; and X4 is a hydrophilic amino acid, such as the sequences IRLPS, IKLPS, LRLPS and LKLPS. The vacuole targeting sequence may be present in a chimeric protein linked to an amino acid sequence of a heterologous protein to facilitate vacuole vacuole targeting of the expressed chimeric protein in a plant cell. The invention is applicable to production of expressed, chimeric proteins in monocots and dicots, and in particular monocots such as cereals and sugarcane. |