| abstract |
Method for the simultaneous detection of adenoviruses of subgenera A, B, C, D, E and F in a sample comprising the steps: a) providing a sample, b) contacting said sample with at least one forward primer and at least one reverse primer having at least 80% identity with a portion of a nucleotide sequence of a hexon gene of an adenovirus of subgenera A, B, C, D, E and F and being derived from sequences SEQ ID No. 1 and/or SEQ ID No. 2 and/or nucleotides 1 to 780 of SEQ ID No. 3 and/or SEQ ID No. 4 and/or SEQ ID No. 5 and/or nucleotides 1 to 780 of SEQ ID No. 6 and/or SEQ ID No. 7 and/or nucleotides 1 to 1200 of SEQ ID No. 8 and/or SEQ ID No. 10 and/or SEQ ID No. 11 and/or nucleotides 1 to 780 of SEQ ID No. 12 and preferably at least one forward primer and at least one reverse primer having at least 80% identity with a portion of a fiber gene of at least one adenovirus of subgenera A, B, C, D, E and/or F, c) subjecting the sample contacted with said primers to a nucleic acid amplification technique, and d) optionally determining the presence of adenoviruses in said sample by detecting a nucleic acid amplification product . |